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MICROSCOPE DIRECTIONS

**I. Preparation of the slide (wet mount)**
1. Place one drop of water on the slide 2. Cut from newspaper a single letter, the lower case “e” and place correct side up in the drop of water 3. Place cover slip on top of specimen. 4. Gently dab off excess water with paper towel

II. Preparing the microscope for use
1. Carrying the microscope- Use two hands one at the base, one on the arm 2. Plug in and turn on light. 3. Open diaphragm to widest, most light setting. 4. Turn to low power objective.

III. The operation of the microscope
1.Place the slide on the stage of the microscope and hold with stage clips 2. Looking at the stage, bring the low power objective as close as possible to the slide using the coarse adjustment. 3. Now, looking through eyepiece slowly focus by moving the coarse adjust so that the stage moves away from the slide. 4. Once focused, use the fine focus and diaphragm to create the best image. 5. Rotate medium or high power objective into place as needed. 6. USE ONLY THE FINE ADJUSTMENT WITH MEDIUM OR HIGH OBJECTIVES.

You must score 100% on this quiz to be authorized to use the microscopes needed in the lab activity.